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Lectin from Pisum sativum (pea)

Name: Lectin from <I>Pisum sativum</I> (pea)
Brand: SIGMA

FITC conjugate, lyophilized powder

Synonym(s):

Pisum sativum agglutinin, PSA

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

Form:

lyophilized powder

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Properties

conjugate

FITC conjugate

Quality Segment

200

form

lyophilized powder

potency

<20 μg per mL agglutination activity

composition

Protein, ≥95% biuret

extent of labeling

2-4 mol FITC per mol protein (Lowry).

impurities

salt, free

storage temp.

−20°C

Description

General description

Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for α-D-glucose and α-D-mannose.

Application

Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns

Biochem/physiol Actions

PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.

Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).

Preparation Note

Conjugates are prepared from affinity purified lectin.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

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This Item	L7381	L0401	L5380
Sigma-Aldrich L0770 Lectin from Pisum sativum (pea)	Sigma-Aldrich L7381 Lectin from Arachis hypogaea (peanut)	Sigma-Aldrich L0401 Lectin from Lycopersicon esculentum (tomato)	Sigma-Aldrich L5380 Lectin from Pisum sativum (pea)
description FITC conjugate, lyophilized powder	description FITC conjugate, lyophilized powder	description FITC conjugate, buffered aqueous solution	description lyophilized powder
form lyophilized powder	form lyophilized powder	form buffered aqueous solution	form lyophilized powder
impurities salt, free	impurities -	impurities -	impurities -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
potency <20 μg per mL agglutination activity	potency -	potency -	potency <20 μg/mL per mL agglutination activity
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate -

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H317,H334

Precautionary Statements

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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