I2658
Anti-phospho-Insulin Receptor Substrate-1 (pTyr612) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Anti-phospho-IRS-1 (pTyr612)
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
human
packaging
vial of 100 μL
technique(s)
western blot: suitable
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr612)
Gene Information
human ... IRS1(3667)
General description
Assay Dependent. It is recommended that the user determine the appropriate working dilution for their process.
Insulin receptor substrate-1 (IRS-1) is among the four IRS proteins (IRS-1-IRS-4) that mediate signalling by insulin receptor (IR) and insulin-like growth factor (IGF) receptor. IRS-1 has a pleckstrin homology domain and a phosphotyrosine-binding domain. . Phosphorylation of Tyr612 and Tyr632 fully activate the PI3K signalling pathway in response to insulin and induce translocation of GLUT4. Thus IRS-1 protein plays a vital role in mediating the metabolic actions of insulin via the PI3K pathway. Additionally, IRS-1 has the abililty to translocate to the nucleus, the mechanism of which is not very clear. The transcriptional activity of IRS-1 is involved in the development of breast and prostate cancers
The antibody recognizes human IRS-1 (pTyr612). It may cross react with in mouse, rat and chicken (100% homologous) IRS-1 (pTyr612).
The antibody recognizes human IRS-1 (pTyr612). It may cross react with in mouse, rat and chicken (100% homologous) IRS-1 (pTyr612).
Immunogen
synthetic phosphopeptide derived from the region of insulin receptor substrate-1 (IRS-1) phosphorylated at tyrosine 612.
Application
Anti-phospho-IRS-1 (pTyr612) antibody may be used for detection by immunoblotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Physical form
Solution in Dulbecco′s PBS, pH 7.3, 50% glycerol containing 1 mg/ml BSA and ≤ 0.1% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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D L Esposito et al.
Endocrinology, 142(7), 2833-2840 (2001-06-21)
To examine contributions of specific YXXM motifs in human insulin receptor substrate-1 (IRS-1) to mediating the metabolic actions of insulin, we studied IRS-1 mutants containing various substitutions of Phe for Tyr. In transfected NIH-3T3(IR) cells, insulin stimulation caused a 5-fold
Vanessa R Haynes et al.
Journal of lipid research, 60(8), 1350-1364 (2019-06-17)
Defects in the gene coding for dysferlin, a membrane-associated protein, affect many tissues, including skeletal muscles, with a resultant myopathy called dysferlinopathy. Dysferlinopathy manifests postgrowth with a progressive loss of skeletal muscle function, early intramyocellular lipid accumulation, and a striking
Susanne Lager et al.
Physiological reports, 2(2), e00242-e00242 (2014-04-20)
As in humans, obesity during pregnancy in mice results in elevated maternal insulin levels and metabolic programming of offspring. mTOR signaling regulates amino acid transport and may function as a placental nutrient sensor. Because obesity is a condition with increased
Byungyong Ahn et al.
The Journal of clinical investigation, 126(9), 3567-3579 (2016-08-09)
Intramuscular lipid accumulation is a common manifestation of chronic caloric excess and obesity that is strongly associated with insulin resistance. The mechanistic links between lipid accumulation in myocytes and insulin resistance are not completely understood. In this work, we used
Amanda M Eccardt et al.
American journal of physiology. Cell physiology, 318(6), C1214-C1225 (2020-04-30)
Reactive oxygen species such as hydrogen peroxide have been implicated in causing metabolic dysfunction such as insulin resistance. Heme groups, either by themselves or when incorporated into proteins, have been shown to scavenge peroxide and demonstrate protective effects in various
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