I2644

Interleukin-2 human

IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-2, hIL-2, Interleukin-2, T-cell growth factor
• MDL number: MFCD00165721
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• form: lyophilized powder
• potency: ≤700 EC₅₀
• quality: endotoxin tested
• mol wt: 15.5 kDa
• packaging: pkg of 5X10 μg; pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P60568
• storage temp.: −20°C
• Gene Information: human ... IL2(3558)

Description

General description

Interleukin-2 (IL-2) is an α-helical cytokine belonging to T-helper cell type 1. It has a molecular weight of 15kDa. The IL2 gene is mapped to human chromosome 4q27. IL-2 is originally called as T cell growth factor.

Application

Interleukin-2 human has been used:

• in the stimulation of natural killer cells
• to study its ability to stimulate lymphocyte growth and prevent apoptosis
• as an antigen in enzyme-linked immunosorbent assay (ELISA), to analyse the human autoantibody reactivities in health and in type 1 diabetes mellitus

Biochem/physiol Actions

Interleukin-2 (IL-2) is essential for proliferation of natural killer (NK) cells, lymphocytes and macrophages. It plays a key role in the immune system functions such as NK cell-stimulated immune responses. IL-2 also mediates the essential functions of regulatory T cells. It is implicated in autoimmune disease. IL-2 is associated with apoptosis and thus, possesses antitumor response. It mediates CD41⁺ T-cells differentiation into T-helper cell 1 and 2 effector subsets, preventing T-helper 17 differentiation.

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lympho­kine promotes long term growth of acti­vated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-inter­feron and B cell growth factor secretion, and modulates the expression of the IL-2 receptor.

Physical form

Lyophilized, 0.2 μm filtered, no additives.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves