GE28-9031-35
Protein G High Performance MultiTrap™
Cytiva 28-9031-35
About This Item
Recommended Products
ligand
recombinant protein G lacking albumin-binding region
material
polypropylene and polyethylene
feature
wetted part: no
packaging
pkg of 4 ea
manufacturer/tradename
Cytiva 28-9031-35
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
30 °C max. temp.
4 °C min. temp.
size
127.8 mm × 85.5 mm × 30.6 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-9
working range
3-9
capacity
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
storage temp.
2-8°C
General description
Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).
A protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min is also provided. No pretreatment of the sample is required.
Features and Benefits
- Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
- Flexible format, protocol for classical elution as well as cross-linking method.
- Formatted for elution for electrophoresis or LC-MS analysis workflows.
- Fast binding kinetics and high capacity provides high yield.
- Easy scale-up with HiTrap® Protein G HP prepacked columns.
- Prepacked with Protein G Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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