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PNGase F Glycosidase

Name: PNGase F Glycosidase
Brand: SIGMA

Removes N-linked glycans, suitable for mass spectrometry, from Elizabethkingia miricola

Synonym(s):

N-Glycosidase F, Peptide N-glycosidase

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About This Item

CAS Number:

83534-39-8

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.5.1.52 (BRENDA, IUBMB)

MDL number:

MFCD06656235

Specific activity:

≥20000 units/mg protein

Biological source:

bacterial (Elizabethkingia miricola)

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Properties

Product Name

PNGase F from Elizabethkingia miricola, buffered aqueous solution

biological source

bacterial (Elizabethkingia miricola)

Quality Segment

200

conjugate

(N-linked)

form

buffered aqueous solution

specific activity

≥20000 units/mg protein

mol wt

36 kDa

shipped in

wet ice

storage temp.

2-8°C

Description

General description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Application

Used to deglycosylate protein.

PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.[1]

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Physical form

Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

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1 of 1

This Item	P7367	G1549	G0535
Sigma-Aldrich G5166 PNGase F from Elizabethkingia miricola	Sigma-Aldrich P7367 PNGase F from Elizabethkingia meningoseptica	Sigma-Aldrich G1549 PNGase F from Elizabethkingia meningoseptica	Sigma-Aldrich G0535 Glycopeptidase A from almonds
biological source bacterial (Elizabethkingia miricola)	biological source bacterial (Elizabethkingia meningoseptica)	biological source -	biological source -
specific activity ≥20000 units/mg protein	specific activity -	specific activity ≥1000 U/mg	specific activity -
form buffered aqueous solution	form powder	form ready-to-use solution	form buffered aqueous glycerol solution
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
mol wt 36 kDa	mol wt ~36 kDa	mol wt ~36 kDa	mol wt -

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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