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product name
Fluoroshield™ with DAPI, histology mounting medium
Quality Level
form
liquid
technique(s)
immunofluorescence: suitable
pH range
7.9-8.3
solubility
water: soluble
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
General description
Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.
Application
Fluoroshield™ with DAPI has been used:
- as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
- as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
- as mounting solution for immunostained heart sections for nuclei detection
Legal Information
FluoroShield is a trademark of ImmunoBioScience Corp.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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