The amplified DNA should be loaded onto an agarose gel after the PCR is completed by adding a separate loading buffer or tracking dye, such as Gel Loading Solution, Catalog Number G2526.
E3004
Extract-N-Amp™ PCR ReadyMix™
Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
Extract-N-Amp™ PCR ReadyMix™
Synonym(s):
Plant direct PCR master mix, Tissue direct PCR master mix
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About This Item
NACRES:
NA.55
UNSPSC Code:
41106303
Pricing and availability is not currently available.
Quality Level
usage
sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications
feature
dNTPs included, hotstart, Direct PCR
technique(s)
PCR: suitable
color
colorless
input
crude DNA
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
General description
Extract-N-Amp™ PCR ReadyMix™ is a specially formulated hot start PCR master mix for direct PCR amplification from the extract.
PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, if additional PCR reactions are required, supplemental PCR ReadyMix may be needed.
Application
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) - | technique(s) PCR: suitable | technique(s) PCR: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| input crude DNA | input - | input purified DNA | input purified DNA |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Huirong Gao et al.
Frontiers in plant science, 11, 535-535 (2020-05-21)
Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing hybrids with multiple traits is expensive, time-consuming, and complex. Here we report using CRISPR-Cas9 to generate a complex
Anneke van der Zee et al.
PloS one, 11(3), e0150755-e0150755 (2016-03-10)
The objective of our study was the development of a semi-quantitative real-time PCR to detect uropathogens. Two multiplex PCR reactions were designed to detect Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter spp., Proteus mirabilis, Enterococcus faecalis, and Pseudomonas aeruginosa. 16S
Xuan Song et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(32), 6378-6394 (2019-06-14)
ATM (ataxia-telangiectasia mutated) is a PI3K-like kinase best known for its role in the DNA damage response (DDR), especially after double-strand breaks. Mutations in the ATM gene result in a condition known as ataxia-telangiectasia (A-T) that is characterized by cancer
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E3004-1.2ML | 04061837692161 |
| E3004-125ML | 04061837692178 |
| E3004-12ML | 04061837692185 |
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Sehr geehrte Damen und Herren, sollen wir nach dem PCR Lauf, Ladepuffer dazugeben?
1 answer-
Helpful?
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Can I use the TriTrack DNA Loading Dye or the TrakitCyanoOrange Loading Buffer with the ReadyMix??
1 answer-
The choice of loading dye will not impact the performance the the E3004 - Extract-N-Amp™ PCR ReadyMix. While we have not tested loading dyes outside of the product, G2526, any commercial DNA loading dye is expected to be compatible.
Helpful?
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What is the polymerase used for this kit? I am trying to calculate Tm for my PCR, but I would need to know what type of polymerase this kit uses. I am guessing something close to Q5 Hotstart?
1 answer-
This kit employs Taq DNA polymerase (Product D6677).
Helpful?
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