E1270
ECM Gel from Engelbreth-Holm-Swarm murine sarcoma
liquid, BioReagent, suitable for cell culture
Synonym(s):
Basement membrane extract (BME), EHS matrix
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About This Item
Recommended Products
product line
BioReagent
Quality Level
form
liquid
concentration
8-12 mg/mL
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Lama1(16772) , Lama3(16774) , Lama5(16776) , Lamb1-1(16777) , Lamc1(226519) , Lamc2(16782) , Nid1(18073) , Sdc2(15529)
Application
ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.
Biochem/physiol Actions
The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.
Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV.
Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV.
Components
ECM was prepared to a protein concentration of 8-12 mg/mL (in DMEM), containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.
Product contains 50 mg/L Gentamicin Sulfate.
Product contains 50 mg/L Gentamicin Sulfate.
Caution
For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.
Preparation Note
This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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