D2916
Lambda DNA Mixed Digest
for DNA electrophoresis
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About This Item
NACRES:
NA.25
UNSPSC Code:
12352200
Pricing and availability is not currently available.
General description
Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Lambda DNA Mixed Digest has been used for DNA electrophoresis.
Preparation Note
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 0.4%.
Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
| form buffered aqueous solution | form liquid | form liquid | form (liquid) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100, 200 | Quality Level 100, 200 | Quality Level 200 | Quality Level 200 |
| usage 100 uses (0.1 mg size) | usage 100 uses (0.1 mg size), 500 uses (0.5 mg size) | usage 100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size) | usage sufficient for 100 loads |
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D2916-.1MG | 04061833562680 |
| D2916-20UG | 04061833562697 |