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H4II

89042702, rat skin, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

H4II, 89042702

biological source

rat skin

growth mode

Adherent

karyotype

52 chromosomes

morphology

Epithelial

products

Albumin, transferrin, prothrombin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat skin Hepatocellular carcinoma

Cell Line Description

From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat . Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. (Same as H4-11-E-C3 (Sigma Catalogue number. 85061112)).

Application

Gene expression analysis.

Culture Medium

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2x100 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 15x1000,000 cells/10cm2 dish.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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