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product name
Grunt Fin (GF), 88010601
biological source
fish fin
growth mode
Adherent
karyotype
Modal no. 47
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Fish Grunt fin tissue blue striped
Cell Line Description
Derived from fin tissue of the adult salt water blue striped Grunt. Does not support the replication of influenza A, herpes simplex, adeno, polio, dengue and eastern equine encephalitis viruses.
Culture Medium
EMEM (HBSS) (Sigma cat no. M5775) + 10mM Hepes (pH 7.3) + 2mM Glutamine + 0.23% (w/v) NaHCO3 + 20% FBS (Heat Inactivated)+ 58mM NaCl
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 20°C. After resuscitation split cells 1:2 to 1:4 and culture for at least one week with 1-2 media changes before shipping. Plating efficiency can be very low a
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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