LA1-55n

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human nerve
• packaging: tube of 5 μg 06041203-DNA-5UG; pkg of vial of cells 06041203-1VL
• growth mode: Semi-adherent aggregates
• karyotype: Not specified
• morphology: Fusiform cells
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Neuroblastoma

Cell Line Description

Derived from LA1-5s a clonal subline of LA-N-1. LA-N-1 was derived from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma.LA-N-1 (Sigma Catalogue number 06041201), LA1-55n (Sigma Catalogue number 06041203) and LA1-5s (Sigma Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

Culture Medium

EMEM (with non-essential amino acids) and Ham′s F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 to 1:100 ratio; 8% CO₂, 37°C. Cells are moderate to weakly adherent. Allow floating clumps to settle then withdraw medium. Remove at

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.