This product is not sterile as received. Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution. We do not recommend sterilizing the collagen solution by membrane filtration as we have found substantial protein loss by this method. Coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes can be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol. Detailed information can be found in the link below:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/collagen-product-protocols
C9301
Chicken Collagen Type II
from chicken sternal cartilage, powder, suitable for cell culture
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Product Name
Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture
biological source
chicken (Sternal cartilage)
Quality Level
type
Type II (Miller)
product line
BioReagent
form
powder
packaging
glass bottle of 100 mg
poly bottle of 25 mg
glass bottle of 5 mg
concentration
60-80% (biuret)
technique(s)
cell culture | mammalian: suitable
surface coverage
6‑10 μg/cm2
solubility
acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
UniProt accession no.
Binding Specificity
Peptide Source: Fibrinogen
shipped in
wet ice
storage temp.
2-8°C
Gene Information
chicken ... COL2A1(395069)
General description
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Collagen is made of three polypeptide chains.[1] Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1[2] chains.[1] Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye.[2] The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.[3]
Application
Collagen from chicken sternal cartilage has been used:
Biochem/physiol Actions
Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.
Features and Benefits
This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.
Other Notes
Major component of articular cartilage.
Preparation Note
Prepared by a modification of the method of Trentham, D.E., et al.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Is this product sterilized? If not, how do we sterilize it? Thank you!
1 answer-
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May I please know the exact molecular weight of the protein ?
1 answer-
The product C9301 gives 1 major band at approximately 150 KDa in the SDS-PAGE test.
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What is the recommended method for storing stock solutions of item C9301, Collagen from chicken sternal cartilage after reconstitution?
1 answer-
Following reconstitution, store the solution in the refrigerator at 4°C. Stock solutions are stable for up to 6 months at 4°C. It is important to avoid freeze/thaw cycles of the solutions.
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