A9688

Anti-Mouse IgM (μ-chain specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Goat Anti-Mouse IgM (μ-chain specific)–AP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162657
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: ELISA (d), IHC (p), WB
• Citations: 23

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: mouse
• technique(s): direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Binds mouse IgM; does not bind other mouse Igs.

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice.
Goat Anti-Mouse IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for mouse IgM when tested against purified mouse IgA, IgG and IgM myeloma proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Goat Anti-Mouse IgM (μ-chain specific)-Alkaline Phosphatase antibody has been used for ELISA and immunofluorescence assays. The antibody can also be used for IHC (1:50) and western blot (1:30,000) applications.

Mouse plasma antibody isotype determination was performed by ELISA using alkaline phopshatase-conjugated goat anti-mouse IgM as the secondary antibody.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)