The concentration of this product is considered to be 100%. Typical usage rates range from 0.005% to 0.01%. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/483/213/a8311pis.pdf
A8311
Antifoam 204
aqueous emulsion for bacterial and mammalian systems
Synonym(s):
organic antifoam
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About This Item
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Quality Segment
form
emulsion (aqueous)
concentration
100% (organic)
density
1.01 g/mL at 25 °C
application(s)
microbiology
storage temp.
room temp
suitability
(Mammalian (suspension)), bacteria (fermentation)
General description
Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.
Application
Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.
Antifoam 204 has been used:
Antifoam 204 has been used:
- in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain[1]
- in fed-batch fermentation
- to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth
- in the batch fermentation with mineral medium
Preparation Note
For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.
Other Notes
Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.
Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly.
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This Item | |||
|---|---|---|---|
| suitability (Mammalian (suspension)), bacteria (fermentation) | suitability (Mammalian (suspension)), bacteria (fermentation) | suitability - | suitability (Mammalian (suspension)), bacteria (fermentation) |
| form emulsion (aqueous) | form emulsion (aqueous) | form liquid | form emulsion (aqueous) |
| application(s) microbiology | application(s) microbiology | application(s) advanced drug delivery | application(s) microbiology |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| density 1.01 g/mL at 25 °C | density 0.97 g/mL at 25 °C | density 1.01 g/cm3 at 25 °C (lit.) | density 1.0 g/mL at 25 °C |
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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What is the concentration of the antifoam 204 aqueous emulsion? Is the recommended concentration 0.005-0.01% (v/v) based on A6426 or A8311?
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