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About This Item
Empirical Formula (Hill Notation):
C12H20N3O14P3 · xNa+
CAS Number:
Molecular Weight:
523.22 (free acid basis)
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.51
Assay:
≥85%
Form:
lyophilized powder
Storage temp.:
−70°C
Pricing and availability is not currently available.
Product Name
Aminoallyl-dUTP sodium salt, ≥85%, lyophilized powder
Quality Segment
assay
≥85%
form
lyophilized powder
shipped in
dry ice
storage temp.
−70°C
SMILES string
[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N
InChI key
YWKTUHBZWBRYHX-TYYBGVCCSA-N
Application
Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
- to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
- for the generation of chromatin immunoprecipitation (ChIP) probes
- in the reverse transcription of RNA for the synthesis of fluorescent labelled probes[1]
Biochem/physiol Actions
Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 100 |
| form lyophilized powder | form lyophilized powder | form powder | form liquid |
| assay ≥85% | assay ≥75% | assay ≥97% | assay ≥95.0% (HPLC) |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
| storage temp. −70°C | storage temp. - | storage temp. −20°C | storage temp. −20°C |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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