94149
Atto 647N-Streptavidin
BioReagent, suitable for fluorescence
Synonym(s):
Streptavidin-Atto 647N
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About This Item
UNSPSC Code:
12161900
NACRES:
NA.25
Recommended Products
product line
BioReagent
Quality Level
form
powder
manufacturer/tradename
ATTO-TEC GmbH
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 650.0-656.0 nm Amax
suitability
corresponds for gel electrophoresis
suitable for fluorescence
storage temp.
−20°C
General description
Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.
Application
Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.
Features and Benefits
Atto 647N- Streptavidin has the following benefits-
- Highly photostable. Strong absorption.
- Excellent fluorescent quantum yield.
- Highly resistant to ozone degradation.
- Minimal triplet formation.
Analysis Note
dye-to-protein ratio ≥1.5
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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