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81349

Sigma-Aldrich

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt

free from low molecular weight nucleotides, lyophilized

Synonym(s):

Poly(dI-dC) sodium salt

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About This Item

CAS Number:
118578-37-3
MDL number:
MFCD00147947
UNSPSC Code:
12352106
NACRES:
NA.52

grade

for molecular biology

form

powder

quality

lyophilized

mol wt

Mr ~2.5 × 106 (broad molecular weight distribution)
Mr ~2500000

packaging

ampule of ≥10 units A260

storage temp.

−20°C

General description

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

Application

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.
Suitable for
  • non-specific blocking agent in EMSA
  • evaluation of DNA methyltransferases
  • DNA-small molecule or drug interaction studies

Biochem/physiol Actions

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition
Dimitrova Y, et al.
Biology Direct, 12(1), 8-8 (2017)
Repeated probing of Southwestern blots using alkaline phosphatase stripping
Jia Y, et al.
Journal of Chromatography A, 1217(45), 7177-7181 (2010)
Xiaomei Xu et al.
eLife, 9 (2020-08-09)
Local activation and long-range inhibition are mechanisms conserved in self-organizing systems leading to biological patterns. A number of them involve the production by the developing cell of an inhibitory morphogen, but how this cell becomes immune to self-inhibition is rather
An enzymatic method for the determination of dCTP and dGTP in picomole amounts.
L Skoog
European journal of biochemistry, 17(2), 202-208 (1970-12-01)
Sebastian Scheer et al.
Nature communications, 10(1), 19-19 (2019-01-04)
Protein methyltransferases (PMTs) comprise a major class of epigenetic regulatory enzymes with therapeutic relevance. Here we present a collection of chemical probes and associated reagents and data to elucidate the function of human and murine PMTs in cellular studies. Our
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