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19359

Glucose dehydrogenase from Pseudomonas sp.

greener alternative

powder, white, ≥200 U/mg

Synonym(s):

GDH

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-836-9
MDL number:
EC Number:
Specific activity:
≥200 U/mg
Biological source:
bacterial (Pseudomonas spp.)
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biological source

bacterial (Pseudomonas spp.)

Quality Segment

form

powder

specific activity

≥200 U/mg

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

color

white

greener alternative category

storage temp.

−20°C

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.

Application

Glucose dehydrogenase from Pseudomonas sp. has been used for the oxidation of glucose as a part of flow-injection analysis detection. It has also been used to regenerate the consumed nicotinamide adenine dinucleotide (NADH) during the removal of pyruvate by lactate dehydrogenase.

Biochem/physiol Actions

In bacteria, the membrane integrated glucose dehydrogenase catalyses the conversion of glucose to gluconic acid. It uses pyrroloquinoline quinone as a coenzyme. The intracellular glucose dehydrogenase lack this cofactor. It is a nicotinamide adenine dinucleotide (NADH) dependent enzyme.

Other Notes

One unit corresponds to the amount of enzyme which will oxidizes 1 μmole β-D-glucose to D-glucono-δ-lactone per minute at pH 8.0 and 37 °C

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1 of 1

This Item
4910110113G3651
biological source

bacterial (Pseudomonas spp.)

biological source

-

biological source

-

biological source

-

specific activity

≥200 U/mg

specific activity

~1 U/mg

specific activity

~120 U/mg

specific activity

≥50 units/mg protein

form

powder

form

powder

form

powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

white

color

dark brown

color

white

color

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Global Trade Item Number

SKUGTIN
19359-10MG-F04061838760852

Questions

1–4 of 4 Questions  
  1. Could this GDH contain PQQ co-factor, or just NADH?

    1 answer
    1. This product is not known to contain PQQ co-factor.

      Helpful?

  2. Does this GDH contain FAD?

    1 answer
    1. No, this product does not contain FAD and is not FAD-dependent. This product is Glucose 1-Dehydrogenase, as such this is an NAD dehydrogenase.

      Helpful?

  3. Does this product contain co-factor NADH?

    1 answer
    1. NADH is already present as a cofactor in this product, so there is no need to add it.

      Helpful?

  4. What solvent can Product 19359, Glucose dehydrogenase be dissolved in? How should it be stored?

    1 answer
    1. Glucose Dehydrogenase can be dissolved in a solvent such as a phosphate buffer or Tris-HCl buffer. The product must be stored in a cool, dry and well-ventilated place with the container tightly closed. Recommended Storage temperature is -20°C.

      Helpful?

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