N4754
Nigrosin water soluble
Powder
Synonym(s):
Acid black 2
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product name
Nigrosin water soluble, For use as a biological stain
Quality Level
form
powder
solubility
water: 1 mg/mL, turbid
εmax
~250 at 575 nm in 50% ethanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
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General description
Nigrosin is an acidic dye used as a negative stain for visualization of the capsule in bacteriological material.
Application
Nigrosin has the following applications:
- to stain live sperm
- as a viable cell staining agent especially in stem cell biology
- as a negative stain in place of India ink for spirochetes, bacteria, protozoa, and fungi
- in Dorner′s spore stain.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm. Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analysis, eosin-nigrosin (EN) staining and the HOS test. A further EN stain was
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