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Sigma-Aldrich

Bromophenol Blue

ACS reagent

Synonym(s):

3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form

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About This Item

Empirical Formula (Hill Notation):
C19H10Br4O5S
CAS Number:
115-39-9
Molecular Weight:
669.96
Beilstein:
61698
EC Number:
204-086-2
MDL number:
MFCD00005875
UNSPSC Code:
12171500
PubChem Substance ID:
24847169
NACRES:
NA.47

grade

ACS reagent

Quality Level

200

form

powder

technique(s)

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

mp

273 °C (lit.)

solubility

ethanol: 0.1%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

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General description

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR7468
BCCM1010
SHBR1340
BCCJ9036
BCCK2682

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