10354074001

Citrate Lyase (CL)

from Klebsiella pneumoniae (Aerobacter aerogenes)

• Synonym(s): Citrate Lyase (CL)
• Enzyme Commission number: 4.1.3.6 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bacterial (Klebsiella pneumoniae)
• Quality Level: 100
• form: lyophilized
• specific activity: ≥0.25 U/mg (At 25 °C with citrate as the substrate.)
• packaging: pkg of 120 U
• manufacturer/tradename: Roche
• technique(s): activity assay: suitable
• color: light cream
• optimum pH: 8.0-9.0
• solubility: water: soluble
• NCBI accession no.: WP_323133469
• UniProt accession no.: P45413
• application(s): life science and biopharma
• foreign activity: ICDH <0.05% (NAD as coenzyme); NADH oxidase <0.05%
• storage temp.: 2-8°C

Description

General description

Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.

Application

It was used in enzymatic assay to measure citrate concentration using microtiter plates.

Quality

Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each

Physical form

Lyophilizate, stabilized with BSA, sucrose, MgSO₄ and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.

Preparation Note

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash