10108090001

Peroxidase (POD)

grade I, from horseradish

• Synonym(s): POD, peroxidase
• UNSPSC Code: 12352204
• EC Number: 1.11.1.7 (BRENDA, IUBMB)
• NACRES: NA.54
• Specific activity: ≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg
• Biological source: horseradish

Properties

• biological source: horseradish
• form: lyophilized (salt-free)
• specific activity: ≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg
• packaging: pkg of 25,000 U
• manufacturer/tradename: Roche
• optimum pH: 6.0-6.5
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Donor:hydrogen-peroxide oxidoreductase

Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.

Application

Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.

Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H₂O₂ produced from disaggregated slug cells.

Biochem/physiol Actions

Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.

Preparation Note

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).

Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.

Analysis Note

Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase
Purity number: approximately 3.0 (A₄₀₃/A₂₇₅)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable