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Millipore

Millipore® Steriflip® Vacuum Tube Top Filter

pore size 0.22 μm, PVDF membrane, funnel capacity 50 mL, pack of 25 ea

Synonym(s):

Steriflip®-GV Sterile Centrifuge Tube Top Filter Unit, tube top sterile filter unit, tube top vacuum filter, tube top vacuum sterile filtering unit

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About This Item

UNSPSC Code:
41104914
eCl@ss:
32031602
NACRES:
NB.22

material

PVDF membrane
acrylic housing
polypropylene

Quality Level

400

sterility

sterile

product line

Steriflip®

feature

holdup volume 0.6 mL
hydrophilic

packaging

pack of 25 ea

parameter

45 °C max. temp.

technique(s)

sterile filtration: suitable

H

138 mm

capacity

50 mL

diam.

6.4 cm

filter diam.

4 cm

filtration area

7 cm2

funnel capacity

50 mL

matrix

Durapore®

pore size

0.22 μm pore size

fitting

double lead thread inlet (with vacuum port)
 double lead thread outlet for 50 mL centrifuge tube

shipped in

ambient

General description

Compatible with 50 mL centrifuge tubes, Steriflip® filters eliminate the liquid transfer step by integrating directly into the sample tube. To filter, attach the unit to the 50 mL centrifuge tube containing your sample, flip, and apply vacuum. The filtrate collects directly into the attached 50 mL tube.

This Steriflip® 0.22 μm pore size polyvinylidene fluoride (PVDF) membrane filter unit combines a 50 mL centrifuge tube with a cap for processing and storage.

Application

For Research Use Only

Steriflip® filters are designed to be used for sterile vacuum filtration of aqueous solutions such as tissue culture media and biological fluids.
  • Tissue culture media +/- additives
  • Buffers
  • Biological solutions
Steriflip® filters have been used:
  • in the preparation of antibody-drug conjugates (ADCs)
  • to sterilize bovine serum albumin (BSA), papain, dispase, and DNase mix and glucose solution for tissue culture
  • to filter clarified supernatant in human immunodeficiency virus (HIV-1) fusion assay

Features and Benefits

  • Eliminates messy sample transfer via a simple vacuum-assisted, closed system
  • Can isolate cells from cellular debris using any of the various pore-sized nylon net filters
  • Ideal for sterilizing small batches of culture media
  • Attaches to any standard 50 mL centrifuge tube, eliminating unnecessary materials, time and risk of spillage

Legal Information

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
Steriflip is a registered trademark of Merck KGaA, Darmstadt, Germany

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Antibody-Drug Conjugates (2013)
Marielle Cavrois et al.
Bio-protocol, 4(16) (2014-08-20)
The HIV-1 fusion assay measures all steps in the HIV-1 life cycle up to and including viral fusion. It relies on the incorporation of a β-lactamase Vpr (BlaM-Vpr) protein chimera into the virion and the subsequent transfer of this chimera
Harish Babu et al.
Frontiers in neuroscience, 5, 89-89 (2011-08-04)
In vitro assays are valuable tools to study the characteristics of adult neural precursor cells under controlled conditions with a defined set of parameters. We here present a detailed protocol based on our previous original publication (Babu et al., 2007)
Jerzy A Woznicki et al.
Cell death & disease, 11(1), 68-68 (2020-01-29)
Proteins of the BCL-2 family are evolutionarily conserved modulators of apoptosis that function as sensors of cellular integrity. Over the past three decades multiple BCL-2 family members have been identified, many of which are now fully incorporated into regulatory networks

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