SCT047
BioTracker Cystine-FITC Live Cell Dye
Live cell imaging dye that measures cystine uptake at the single cell level used to detect glutathione (GSH) synthesis, reactive oxygen species (ROS) detoxification, T-cell activation and B-cell development.
Synonym(s):
Cellular cystine uptake detection, Live cell imaging probe
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.47
Recommended Products
Assay
≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
General description
Uptake of cystine allows accumulation of cysteine that is necessary for glutathione synthesis and reactive oxygen species (ROS) detoxification. After the uptake of cystine into the cytoplasm, the reducing intracellular environment leads to a separation of one cystine molecule into two cysteine molecules. A key function of cysteine is to maintain the cellular pools of glutathione (GSH). Cystine uptake is also required for T-cell activation and B-cell development.
The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods.
Spectral Properties
Absorbance: 450nm
Emission: 525nm
The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods.
Spectral Properties
Absorbance: 450nm
Emission: 525nm
Application
Live cell fluorescent imaging
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Quality
Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 625.65 g/mol
Molar Mass: 625.65 g/mol
Physical form
Lyophilized
Storage and Stability
Store BioTracker Cystine-FITC Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Peter J Siska et al.
Journal of immunological methods, 438, 51-58 (2016-09-07)
T and B lymphocytes undergo metabolic re-programming upon activation that is essential to allow bioenergetics, cell survival, and intermediates for cell proliferation and function. To support changes in the activity of signaling pathways and to provide sufficient and necessary intracellular
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