SCC440

SK-MEL-147 Human Melanoma Cell Line 

Human

• Synonym(s): Q61R NRAS melanoma cell line, SK-MEL-147, human melanoma cell line, human melanoma cells, metastatic melanoma cell line
• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• product name: SK-MEL-147 Human Melanoma Cell Line ,
• biological source: human
• Quality Level: 100
• packaging: vial of ≥1X10⁶ vial
• manufacturer/tradename: Millipore
• technique(s): cell culture | mammalian: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Description

General description

SK-MEL-147 is a human metastatic melanoma cell line. It retains a Q61R NRAS point mutation which is associated with a more aggressive phenotype, higher metastasis risk, and poorer prognosis.

Application

• Each vial contains >1X10⁶ viable cells.
• SK-MEL-147 cells are verified to be of human origin and negative for mouse, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells tested negative for infectious diseases against a Contamination Clear panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma.

Features and Benefits

The SK-MEL-147 cell line expresses PMEL and MelanA proteins, two common melanoma markers. PMEL has been implicated in poor immune cell infiltration, and may be a relevant target for drug therapy due to its limited expression in normal cell phenotypes.

Target description

Melanoma is considered the most dangerous form of skin cancer. It develops in melanocytes, the pigment producing cells of the skin. Melanoma accounts for a very small number of all skin cancers. Even though it accounts for only about 1% of total diagnosed

Storage and Stability

SK-MEL-147 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting cell marker expression and functionality.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact l

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable