MABT1489

Anti-Kinesin Light Chain Antibody, clone 63-90

clone 63-90, from mouse

• Synonym(s): KLC1, KLC2, KLC
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 63-90, monoclonal
• species reactivity: squid, rat, mouse
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001075443
• UniProt accession no.: B2GV74; P37285
• target post-translational modification: unmodified
• Gene Information: rat ... Klc1(171041)

Description

General description

Kinesin light chain (UniProt: P37285 and B2GV74 for KLC1 and KLC2, respectively) is encoded by the Klc1/Klc2 (also known as Klc, Kns2) gene (Gene ID: 171041/309159) in rat. Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The kinesin light chain (KLC) may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity. Kinesin light chain 1 is expressed in the brain tissue and exists as an oligomeric complex of two heavy and two light chains. It also contains six tetratricopeptide repeats (TPR). The heavy chains of kinesin have a microtubule-binding ATPase motor domain at their amino terminus, a neck coil, and an extended series of coiled coils, separated by a hinge region, that results in heavy-chain dimerization. Kinesin light chains can associate with the heavy-chain coiled coils at the carboxyl-terminal region. The light chains bind cargoes and regulate its activity. KLCs through their TRP repeat domains can recognize shirt linear peptide motifs found on cargo proteins. In the absence of any cargo binding, kinesin-1 exists in a folded compact state, which prevents unnecessary cycles of ATP hydrolysis. However, in the cargo-bound active state inhibition is relieved and it adopts a more elongated structure that can hydrolyze ATP and translocate along microtubules. Point mutations in Klc gene can affect functional domains in kinesin, which can result in late-onset dying-back neuropathies in sensory or motor neurons. (Ref.: Yip, YY., et al. (2016). Proc. Natl. Acad. Sci. USA 113(9); 2418-2423; Pigino, G., et al. (2009). Proc. Natl. Acad. Sci. USA 106(14); 5907-5912).

Specificity

Clone 63-90 detects both KLC1 and KLC2. It targets an epitope with in the N-terminal region.

Immunogen

Purified rat brain Kinesin light chain 1.

Application

Anti-Kinesin Light Chain, clone 63-90, Cat. No. MABT1489, is a mouse monoclonal antibody that detects Kinesin light chain and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Kinesin Light Chain in rat cerebral cortex tissue sections.

Western Blotting Analysis: 4 µg/mL from a representative lot detected Kinesin Light Chain in rat brain tissue lysate.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Kinesin Light Chain in Immunoprecipitation applications (Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89).

Immunohistochemistry Analysis: A representative lot detected Kinesin Light Chain in Immunohistochemistry applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403).

Western Blotting Analysis: A representative lot detected Kinesin Light Chain in Western Blotting applications (Rahman, A., et. al. (1998). J Biol Chem. 273(25):15395-403; Stenoien, D., et. al. (1997). Mol Biol Cell. 8(4):675-89; Pigino, G., et. al. (2009). Proc Natl Acad Sci U S A. 106(14):5907-12; Pigino, G., et. al. (2003). J Neurosci. 23(11):4499-508).

Research Category
Cell Structure

Quality

Evaluated by Immunohistochemistry (Paraffin) in rat hippocampus tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Kinesin Light Chain in rat hippocampus tissue sections.

Target description

~62 and 70 kDa observed for KLC1 and KLC2, respectively; 63.75 and 68.61 calculated for KLC1 and KLC2, respectively. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable