CD14 is composed of 356 amino acids and is anchored to the cell membrane by linkage to glycosyl phosphatidylinositol. In addition to the 53-55 kDa cell surface form, two soluble forms have been described (Goyert, 2002). CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes (Goyert, 2002). Most tissue macrophages and Langerhans cells also express CD14 (Goyert, 1999 and 2002; Gadd, 1989). Anti-CD14, TUK4, was included in the Fourth, Fifth and Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD14 (Goyert 1995 and 1996).
Specificity
Antibody recognizes Human CD14 antigen.
Application
Research Category Inflammation & Immunology
Research Sub Category Immunoglobulins & Immunology
This Milli-Mark Anti-CD14 -FITC Antibody, clone TUK4 is validated for use in FC for the detection of CD14.
Quality
Evaluated by flow cytometry using human PBMCs.
Target description
53-55 kDa (Goyert, 2002)
Physical form
Protein A purified
Purified Mouse monoclonal IgG2a conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Storage and Stability
Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
Analysis Note
Control PBMCs
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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