ASCIZ is a transcription factor that interacts with the potent kinase ATM and regulates its activity and stability. ATM is best known for its role as an apical activator of the DNA damage response in the face of DNA double-strand breaks (DSBs). Following induction of DSBs, ATM mobilizes one of the most extensive signaling networks that responds to specific stimuli and modifies directly or indirectly a broad range of targets. ASCIZ plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage as well. ASCIZ is also critical in development. Knockouts are lethal at E16 and show severe organ developmental defects, including a complete absence of lungs similar to mutants in Wnt2-2b/ß-catenin and FGF10/FGFR2b signaling pathways. Thus, ASCIZ has dual functions as an efficiency factor for DNA base damage repair as well as a key transcriptional regulator of early lung development. ASCIZ is localized to the nucleus in discrete foci during G1 and is ubiquitously expressed in normal and cancerous tissues.
Immunogen
Epitope: Required for formation of RAD51 foci
KLH-conjugated linear peptide corresponding to human ASCIZ required for formation of RAD51 foci.
Application
Anti-ASCIZ is an antibody against ASCIZ for use in Western Blotting.
Quality
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ASCIZ in 10 µg of human skeletal muscle tissue lysate.
Target description
~100/120 kDa observed. Uncharacterized band(s) may appear in some lysates.
Other Notes
Concentration: Please refer to lot specific datasheet.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The Journal of experimental medicine, 219(9) (2022-07-27)
Disease relapse and treatment-induced immunotoxicity pose significant clinical challenges for patients with hematological cancers. Here, we reveal distinctive requirements for neutralizing TNF receptor ligands APRIL and BAFF and their receptor activity in MM and DLBCL, impacting protein translation and production
Questions
Reviews
★★★★★ No rating value
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
![[3-(Methacryloylamino)propyl]trimethylammonium chloride solution 50 wt. % in H2O](/deepweb/assets/sigmaaldrich/product/structures/189/736/089bc8ae-2a98-416d-9f9a-a0a510b6b828/640/089bc8ae-2a98-416d-9f9a-a0a510b6b828.png)
![[2-(Methacryloyloxy)ethyl]trimethylammonium chloride solution 75 wt. % in H2O](/deepweb/assets/sigmaaldrich/product/structures/316/612/66b0f4cf-d060-427d-b4f5-e8fab3e5cffe/640/66b0f4cf-d060-427d-b4f5-e8fab3e5cffe.png)
