AB1761-I
Anti-Protein Gene Product 9.5 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1
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All Photos(7)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, rat
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), equine (based on 100% sequence homology), porcine (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... UCHL1(7345)
General description
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UniProt: P09936; also known as EC: 3.4.19.12, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1) is encoded by the UCHL1 gene (Gene ID: 7345) in human. UCH-L1 is a member of the peptidase C12 family that can exist as a monomer or homodimer. It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors and is detected in neuronal cell bodies and processes throughout the neocortex. It displays a weak expression in ovary. About 30% of total UCH-L1 is reported to be associated with membranes in brain. Its levels are shown to be down-regulated in brains from Parkinson disease and Alzheimer disease patients. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin and is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimeric form may have ATP-independent ubiquitin ligase activity. Mutations in UCHL1 gene are known to cause Parkinson disease 5, a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Some mutations in this gene are also linked to spastic paraplegia 79, where patients display a form of spastic paraplegia, which is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Immunogen
KLH-conjugated linear peptide corresponding to human Protein Gene Product 9.5.
Application
Detect PGP9.5 using this rabbit polyclonal antibody, Anti-Protein Gene Product 9.5 Antibody validated for use in western blotting, IHC (Paraffin), Immunofluorescence & ICC.
Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected Gene Product 9.5 in human cerebellum.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Gene Product 9.5 in human cerebellum.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Gene Product 9.5 in E18 rat cortical cells..
Dylight® is a registered trademark of Thermo Fisher Scientific.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Gene Product 9.5 in human cerebellum.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Gene Product 9.5 in E18 rat cortical cells..
Dylight® is a registered trademark of Thermo Fisher Scientific.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Quality
Evaluated by Western Blotting in rat brain, human brain, and mouse brain tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Protein Gene Product 9.5 (UCH-L1) in rat brain, human brain, and mouse brain tissue lysate
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Protein Gene Product 9.5 (UCH-L1) in rat brain, human brain, and mouse brain tissue lysate
Target description
~24 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Linkage
Replaces: AB1761 and AB9724
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH7.4 with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
DyLight is a registered trademark of Pierce Biotechnology, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Alexandra B Pincus et al.
Scientific reports, 12(1), 5006-5006 (2022-03-25)
We report subpopulations of airway parasympathetic neurons expressing substance P, neuronal nitric oxide synthase, and tyrosine hydroxylase, highlighting unexplored heterogeneity in this population. These neurotransmitter-specific subpopulations did not form intraganglionic interneurons, but rather, extended outside the ganglia, into the airways
Rashmi Chandra et al.
JCI insight, 8(23) (2023-12-08)
Epidemiological and histopathological findings have raised the possibility that misfolded α-synuclein protein might spread from the gut to the brain and increase the risk of Parkinson's disease. Although past experimental studies in mouse models have relied on gut injections of
Becky J Proskocil et al.
Journal of neuroinflammation, 18(1), 209-209 (2021-09-18)
Toll-like receptor 7 (TLR7) is an innate immune receptor that detects viral single-stranded RNA and triggers the production of proinflammatory cytokines and type 1 interferons in immune cells. TLR7 agonists also modulate sensory nerve function by increasing neuronal excitability, although
Yuan Hu et al.
Cellular and molecular life sciences : CMLS, 80(4), 106-106 (2023-03-24)
Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is a unique component of the ubiquitin-proteasome system (UPS), which has multiple activities in maintaining intracellular ubiquitin levels. We previously reported the aberrant low expression of UCHL1 in podocytes of non-immune complex-mediated glomerulonephritis, and recent
Daniela M Menichella et al.
Molecular pain, 12 (2016-09-04)
Small fiber neuropathy is a well-recognized complication of type 2 diabetes and has been shown to be responsible for both neuropathic pain and impaired wound healing. In previous studies, we have demonstrated that ganglioside GM3 depletion by knockdown of GM3
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