Single colonies can be achieved with the proper dilution of the cells, and efficient spreading across the plate.
71013-M
ColiRollers® Plating Beads
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352202
Pricing and availability is not currently available.
manufacturer/tradename
Novagen®
Quality Segment
storage condition
OK to freeze
shipped in
ambient
storage temp.
15-25°C
General description
ColiRollers™ Plating Beads offer a better way to perform a routine procedure-plating bacteria. Typical plating methods use a bent glass rod "hockey stick," which must be immersed in 70% ethanol and flamed between uses, or disposable plastic applicators. Both require vigorous spreading while rotating the plate. These older methods can become extremely laborious and time consuming when processing many samples, and can produce inconsistent results due to differences in pressure applied, length of rotation time, and moisture content of the plates. In addition, an open flame can present a safety hazard. Novagen ColiRollers Plating Beads are specially treated glass beads that do the work for you. Simply pipet the cells on the plate, add 10 to 20 beads, and move the plate back and forth a few times. The rolling action of the beads gently spreads the cells evenly and completely over the agar surface. Entire stacks of plates can be processed at one time. After plating, shake the beads off the plate and the plate is ready for the incubator. Use of ColiRollers beads eliminates the inconsistencies of conventional "hockey stick" methods, and provides even distribution of colonies every time. ColiRollers beads are provided sterile and each package contains enough beads for 60 to 80 plates.
Legal Information
ColiRollers is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity- Standard Handling
Storage Class
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Is it possible to obtain single colonies with this method? Of course this is also dependent on dilution factors etc, but I am wondering if the beads make enough smear to isolate single colonies.
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Hello Sir, I want to order coliRollers plating beads with catalogue no (71013-M). I want to get the details of this product, like how many grams or beads are there in a package. Thank you.
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The number of beads in each package will vary slightly as the packages are based on weight. The weight of each package is approximately 71g. The recommend number of beads per plate is 10 to 20. Each package contains enough beads for 60 to 80 plates.
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Hi, I would like to ask, can the plating beads be reused and autoclavable?
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The ColiRollers beads are delivered sterile (gamma irradiated) and can be used directly. After use, the ColiRollers can be autoclaved and reused. Used beads can be stored in 75% ethanol solution or 10% bleach solution until autoclaving.
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Can these beads be reused by soaking in ethanol and/or autoclaving?
1 answer-
These beads are sterile (gamma irradiated) and ready to use. This product may also be autoclaved for repeated usage. Beads may also be stored in 75% ethanol or 10% bleach solution.
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What is the size of the beads? 3 mm or 1 mm?
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The approximate size of the beads is 4 mm.
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