666133

Urease, Canavalia ensiformis

Native urease from Canavalia ensiformis. Catalyzes hydrolysis of urea to carbon dioxide and ammonia. Useful in the determination of urea in body fluids. Note: 1 KU = 1000 units.

• Synonym(s): Urease, Canavalia ensiformis
• CAS Number: 9002-13-5
• Enzyme Commission number: 3.5.1.5 (BRENDA, IUBMB)
• MDL number: MFCD00070858
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: Canavalia ensiformis
• Quality Level: 100
• form: lyophilized solid
• specific activity: >150 units/mg material (Nesslers)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: 0.05 M Tris-HCl/0.001 M EDTA, pH 8.0: 5 mg/mL
• cation traces: NH₄⁺: ≤4 μmol/U (μmol/U enzyme)
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native urease from Canavalia ensiformis. Catalyzes hydrolysis of urea to carbon dioxide and ammonia. Useful in the determination of urea in body fluids. Note: 1 KU = 1000 units.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will release 1 µmol of ammonia from urea per min at 25°C, pH 7.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1