444256

MMP Substrate III, Fluorogenic

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination.

• Synonym(s): MMP Substrate III, Fluorogenic, DABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
• Empirical Formula (Hill Notation): C₆₃H₈₈N₁₆O₁₄S
• Molecular Weight: 1325.54
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: red
• solubility: DMSO: soluble; Tris-buffered saline, pH 7.6: soluble
• fluorescence: λ_ex ~340 nm; λ_em ~485 nm
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (k_cat/K_m = 619,000 M^-1s^-1 for MMP-2, 206,000 M^-1s^-1 for MMP-9, 40,000 M^-1s^-1 for MMP-3, 21,000 M^-1s^-1 for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [k_cat/K_m = 619,000 M^-1s^-1 for MMP-2, 206,000 M^-1s^-1 for MMP-9, 40,000 M^-1s^-1 for MMP-3, and 21,000 M^-1s^-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Detection of MMP activity

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

DABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Beekman, B., et al. 1996. FEBS Lett390, 221.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable