No, Brilliant Cresyl Blue is intended for use with fresh peripheral blood. For the staining to occur, the blood cells must be viable. OCT frozen sections or paraffin sections are not viable tissues.
1.01384
Brilliant cresyl blue solution
for the staining of reticulocytes for microscopy
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About This Item
NACRES:
NA.21
UNSPSC Code:
41116121
Pricing and availability is not currently available.
Quality Segment
form
liquid
IVD
for in vitro diagnostic use
technique(s)
microbe id | staining: suitable
pH
3.7 (20 °C in H2O)
density
1.01 g/cm3 at 20 °C
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
General description
The Brilliant cresyl blue solution - for the staining of reticulocytes - for microscopy, is a ready-to-use solution used for human-medical cell diagnosis and serves the hematological investigation of sample material of human origin.
The dye stains the nucleic acids in the reticulocytes (Substantia granulofilamentosa), thus enabling reticulocytes to be morphologically distinguished from erythrocytes. The Substantia granulofilamentosa (ribonucleoproteins) can be rendered visible with fresh, non-fixed, young erythrocytes in a supervital staining procedure. Characterized by an intense black-blue hue, it is possible both to determine the number of the reticulocytes (visual microscopic counting) and also to visualize their morphological aspects. Four stages of substantia granulofilamentosa maturation can be distinguished depending on the stage of reticulocyte development: coiled skein (I), incomplete network (II), complete network (III) and granular form (IV). In peripheral blood the development stages III and IV are found most commonly.
The 100 ml bottle provides ca. 1000 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
The dye stains the nucleic acids in the reticulocytes (Substantia granulofilamentosa), thus enabling reticulocytes to be morphologically distinguished from erythrocytes. The Substantia granulofilamentosa (ribonucleoproteins) can be rendered visible with fresh, non-fixed, young erythrocytes in a supervital staining procedure. Characterized by an intense black-blue hue, it is possible both to determine the number of the reticulocytes (visual microscopic counting) and also to visualize their morphological aspects. Four stages of substantia granulofilamentosa maturation can be distinguished depending on the stage of reticulocyte development: coiled skein (I), incomplete network (II), complete network (III) and granular form (IV). In peripheral blood the development stages III and IV are found most commonly.
The 100 ml bottle provides ca. 1000 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Application test (Reticulocyte staining): passes test
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hi there, I would like to ask regarding the ready-to-use solution Brilliant Cresyl Blue Solution. Is this something can be used on tissue sections and if yes, what is the proper setting? Is it tested on OCT frozen sections or paraffin embedded sections?
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