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MilliporeSigma
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Key Documents

1.00898

Millipore

E.coli / Coliform Selective-Supplement

for Escherichia coli, for coliforms, pkg of 10 vials, for use with ChromoCult® Coliform Agar

Synonym(s):

E. coli / Coliform supplement, E. coli / Coliform antibiotic Selective Supplement

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.74

form

lyophilized powder

Quality Level

200

composition

Cefsulodine, 2.5 mg per vial
Vancomycin, 2.5 mg per vial

packaging

pkg of 10 vials

technique(s)

microbiological culture: suitable

pH

3.5-4.5 (25 °C, 14 g/L in H2O)

application(s)

food and beverages
microbiology

compatibility

for use with ChromoCult® Coliform Agar

storage temp.

2-8°C

suitability

Escherichia coli
coliforms

Related Categories

General description

E. coli / Coliform supplement is a mixture of two antibiotics in lyophilized form. Vancomycin inhibits the growth of Gram-positive bacteria. Cefsulodin suppresses the growth of Pseudomonas spp. and Aeromonas spp. This selective supplement can be used in combination with most E. coli / Coliform enrichment media and plating agars.

Application

E. coli / Coliform Selective-Supplement is an additive for the preparation of selective culture media for the detection of E. coli / Coliforms in water, food, and environmental samples.

Compatibility

For use with Chromocult® Coliform Agar (1.10426)

Linkage

Replaces: 1.00898

Analysis Note

Supplement tested in Chromocult Coliform agar art. no. 1.10426.
Recovery on test medium
- Escherichia coli DSM 502:
≥ 70 %
- Citrobacter freundii ATCC 8090: ≥ 70 %
Growth
- Aeromonas hydrophila ATCC 7966 (WDCM 00063):
inhibited
- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025): inhibited
- Bacillus licheniformis ATCC 14580 (WDCM 00068): inhibited
- Staphylococcus aureus ATCC 6538 (WDCM 00032): inhibited
Colony colour
- Escherichia coli DSM 502:
blue
- Citrobacter freundii ATCC 8090: red
Incubation: 24 hrs.; 35 °C; aerobic.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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