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Key Documents

07-690

Sigma-Aldrich

Anti-Histone H3 Antibody, CT, pan

serum, Upstate®

Synonym(s):

H3, Histone H3, H3 histone family, member T, histone 3, H3, histone cluster 3, H3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse, yeast, rat, chicken, Saccharomyces cerevisiae

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Broad species cross-reactivity expected due to sequence homology.
Recognizes C-Terminal region of Histone H3, Mr 17 kDa

Immunogen

KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.

Application

Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.
Use Anti-Histone H3 Antibody, CT, pan (rabbit polyclonal antibody) validated in ChIP, WB to detect Histone H3 also known as H3, Histone H3, H3 histone family member T, histone 3 H3, histone cluster 3 H3.

Quality

Routinely evaluated by immunoblot on acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Target description

Approx. 17 kDa

Physical form

Rabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Reduced hepatic stellate cell expression of Kruppel-like factor 6 tumor suppressor isoforms amplifies fibrosis during acute and chronic rodent liver injury.
Ghiassi-Nejad, Z; Hernandez-Gea, V; Woodrell, C; Lang, UE; Dumic, K; Kwong, A; Friedman, SL
Hepatology null
Lauren J Buro et al.
Epigenetics & chromatin, 3(1), 16-16 (2010-09-10)
Signal transducer and activator of transcription (STAT) activation of gene expression is both rapid and transient, and when properly executed it affects growth, differentiation, homeostasis and the immune response, but when dysregulated it contributes to human disease. Transcriptional activation is
A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.
Kizer, Kelby O, et al.
Molecular and cellular biology, 25, 3305-3316 (2005)
Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.
Xiao, Tiaojiang, et al.
Genes & Development, 17, 654-663 (2003)
Evelina Y Basenko et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(46), E6339-E6348 (2015-11-19)
H3K9 methylation directs heterochromatin formation by recruiting multiple heterochromatin protein 1 (HP1)-containing complexes that deacetylate histones and methylate cytosine bases in DNA. In Neurospora crassa, a single H3K9 methyltransferase complex, called the DIM-5,-7,-9, CUL4, DDB1 Complex (DCDC), is required for

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