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Key Documents

06-1002

Sigma-Aldrich

Anti-LAP2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

LEM domain containing 4, TP alpha, TP beta/gamma, TPRP isoform alpha, TPRP isoforms beta/gamma, Thymopoietin isoform alpha, Thymopoietin, isoforms beta/gamma, Thymopoietin-related peptide isoform alpha, Thymopoietin-related peptide isoforms beta/gamma, l

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse, canine

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

General description

The inner membrane of the nuclear envelope is connected to the nuclear lamina. The main components of the nuclear lamina are Type V intermediate filaments called lamins and lamin binding proteins including lamin B receptor, emerin, MANI, LAP1 (3 isoforms), and LAP2 (several isoforms). The lamins are divided into two groups: the constitutively expressed lamin B and the developmentaly regulated lamin A.
The best characterized isoforms of lamin binding protein LAP2 are LAP2α and LAP2β. LAP2β is a type II membrane protein in the inner nuclear membrane that binds lamin B and is important to cell viability and controlling nuclear lamina growth. LAP2α has been characterized as a nucleoplasmic protein that interacts with A-type lamins to control gene expression, transcription, and chromatin organization.

Specificity

Predicted to cross-react with canine (dog) and rat based on sequence homology.
This antibody recognizes LAP2.

Immunogen

KLH-conjugated synthetic linear peptide corresponding to LAP2.

Application

Anti-LAP2 Antibody is an antibody against LAP2 for use in WB, IC & IF.
Immunofluorescence:
Representative lot data. This antibody was used to detect the nuclear lamina by immunofluorescence.

Immunocytochemistry:
Representative lot data.
A previous lot was used in confocal fluorescent analysis of A431, HeLa and NIH/3T3 cells using anti-LAP2 rabbit polyclonal antibody (Red).
Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by western blot on HeLa cell lysate.

Western Blot Analysis:
A 1:1000-1:3000 dilution of this antibody was used to detect LAP2 in HeLa cell lysate.

Target description

~55 kDa

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
HeLa cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Anna M Chizhik et al.
ACS nano, 11(12), 11839-11846 (2017-09-19)
The nuclear envelope, comprising the inner and the outer nuclear membrane, separates the nucleus from the cytoplasm and plays a key role in cellular functions. Nuclear pore complexes (NPCs), which are embedded in the nuclear envelope, control transport of macromolecules
Alexey I Chizhik et al.
Methods in molecular biology (Clifton, N.J.), 2175, 33-45 (2020-07-19)
The nuclear envelope, comprising the inner and the outer nuclear membrane, separates the nucleus from the cytoplasm and plays a key role in cellular functions. Nuclear pore complexes (NPCs) are embedded in the nuclear envelope and control transport of macromolecules
Nadia Korfali et al.
PloS one, 6(4), e18762-e18762 (2011-05-03)
Disruption of cell cycle regulation is one mechanism proposed for how nuclear envelope protein mutation can cause disease. Thus far only a few nuclear envelope proteins have been tested/found to affect cell cycle progression: to identify others, 39 novel nuclear
Nadia Korfali et al.
Methods in molecular biology (Clifton, N.J.), 1411, 3-44 (2016-05-06)
Nuclei can be relatively easily extracted from homogenized liver due to the softness of the tissue and crudely separated from other cellular organelles by low-speed centrifugation due to the comparatively large size of nuclei. However, further purification is complicated by
Nadia Korfali et al.
Nucleus (Austin, Tex.), 3(6), 552-564 (2012-09-20)
One hypothesis to explain how mutations in the same nuclear envelope proteins yield pathologies focused in distinct tissues is that as yet unidentified tissue-specific partners mediate the disease pathologies. The nuclear envelope proteome was recently determined from leukocytes and muscle.

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