L020000

TCA Deblock

• UNSPSC Code: 41116004
• NACRES: NA.21

Properties

• type: for DNA synthesis; for DNA synthesis
• Quality Level: 200
• product line: Proligo Reagents
• form: liquid
• storage condition: dry at room temperature
• concentration: 28-32 g/L (TCA content, titration)
• technique(s): DNA synthesis: suitable
• impurities: ≤200 ppm water content (Karl Fischer)
• color: clear; clear
• bp: 39.8—40.0 °C/1,013 hPa
• storage temp.: room temp

Description

Preparation Note

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Other Notes

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H315,H318,H335,H336,H351,H411
• Precautionary Statements: P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Central nervous system, Respiratory system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Flash Point(F): 212.0 °F - closed cup
• Flash Point(C): 100 °C - closed cup