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p53 regulates a G2 checkpoint through cyclin B1.

Proceedings of the National Academy of Sciences of the United States of America (1999-03-03)
S A Innocente, J L Abrahamson, J P Cogswell, J M Lee
摘要

The p53 tumor suppressor controls multiple cell cycle checkpoints regulating the mammalian response to DNA damage. To identify the mechanism by which p53 regulates G2, we have derived a human ovarian cell that undergoes p53-dependent G2 arrest at 32 degrees C. We have found that p53 prevents G2/M transition by decreasing intracellular levels of cyclin B1 protein and attenuating the activity of the cyclin B1 promoter. Cyclin B1 is the regulatory subunit of the cdc2 kinase and is a protein required for mitotic initiation. The ability of p53 to control mitotic initiation by regulating intracellular cyclin B1 levels suggests that the cyclin B-dependent G2 checkpoint has a role in preventing neoplastic transformation.

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抗-磷酸化-Ser/Thr-Pro,MPM-2抗体, clone MPM-2, Upstate®, from mouse