产品名称
Ascentis® Express F5, 2 μm UHPLC 色谱柱, 2 μm particle size, L × I.D. 7.5 cm × 3 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L43
产品线
Ascentis®
特点
endcapped
制造商/商品名称
Ascentis®
包装
1 ea of
参数
1000 bar max. pressure (14500 psi)
60 °C temp. range
技术
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
长度 × 内径
7.5 cm × 3 mm
表面积
120 m2/g
杂质
<5 ppm metals
基质
Fused-Core particle platform
superficially porous particle
基质活性基团
PFP (pentafluorophenyl) phase
粒径
2 μm
孔径
90 Å pore size
工作pH值
2-8
应用
food and beverages
分离技术
reversed phase
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一般描述
Ascentis® Express 2.0 μm F5是基于Fused-Core®颗粒设计的快速UHPLC柱。这种颗粒设计在0.4 μm厚的多孔壳中的扩散路径较浅，且总体粒径小至2 μm，因此柱效很高。Ascentis® Express 2.0 μm F5的键合封端的二甲基五氟苯基丙基固定相由于存在负电性氟而可提供含缺电子苯环的稳定的反相填充。除了形成π-π相互作用和温和的空间相互作用外，F5相还通过极性相互作用保留化合物。F5相具有极性和非极性特性，可表现出双模式保留行为，有时会随流动相中乙腈含量的变化而产生“ U形”保留，在ACN浓度低和高的情况下保留都会增加（ 反相和HILIC保留模式）。Ascentis® Express 2.0 μm F5可作为C18选择性的补充，对碱性、酸性或中性化合物进行分析。
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Jan Soukup et al.
Journal of chromatography. A, 1374, 102-111 (2014-12-30)
Excess adsorption of water from aqueous acetonitrile mobile phases was investigated on 16 stationary phases using the frontal analysis method and coulometric Karl-Fischer titration. The stationary phases include silica gel and silica-bonded phases with different polarities, octadecyl and cholesterol, phenyl
Imran Ali et al.
Journal of separation science, 35(23), 3235-3249 (2012-11-28)
New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate
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