推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1B2, recombinant monoclonal
描述
1B2 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 28.52 kDa
observed mol wt ~32 kDa
純化經由
using Protein A
物種活性
human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
表位序列
C-terminus
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... ELANE(1991)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1B2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects human Neutrophil elastase. It targets an epitope within 14 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Neutrophil Elastase.
應用
Quality Control Testing
Evaluated by Western Blotting in U937 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neutrophil Elastase in U937 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Neutrophil Elastase in lysates from THP-1 cells and Human bone marrow.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Neutrophil Elastase in human bone marrow tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Neutrophil Elastase with a KD of 5.6 x 10-7 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Neutrophil Elastase in one million THP-1 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Neutrophil Elastase in THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in U937 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neutrophil Elastase in U937 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Neutrophil Elastase in lysates from THP-1 cells and Human bone marrow.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Neutrophil Elastase in human bone marrow tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Neutrophil Elastase with a KD of 5.6 x 10-7 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Neutrophil Elastase in one million THP-1 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Neutrophil Elastase in THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Neutrophil elastase (UniProt: P08246; also known as EC:3.4.21.37, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase) is encoded by the ELANE (also known as ELA2) gene (Gene ID: 1991) in human. Neutrophils are the predominant circulating leukocyte population and are important in modulating innate and adaptive immunity. They serve as early responders and are recruited to sites of inflammation and infection by environmental cues. Neutrophil elastase (NE), a member of the peptidase S1 family, is synthesized with a signal peptide (aa 1-27) and a short propeptide (aa 28-29) that are subsequently cleaved off to produce the mature NE (aa 30-267). The mature form contains two glycans and four disulfide bonds. Release of NE can also stimulate adverse remodeling by degrading virtually all the components of the ECM in addition to elastin, and including collagen, fibronectin, and laminin. It is reported to cleave the triple-helix structure of type III and IV collagens, and cross-linked regions of type I, II, and V collagens, aggrecans, and fibronectin. NE-induced degradation of extracellular matrix (ECM) can release bioactive peptides and growth factors such as EGF and FGF that have mitogenic and motogenic effects on smooth muscle cells and fibroblasts. Increased activation of NE can lead to activation of matrix metalloproteinases that could potentiate ECM degradation. NE is also reported to cleave CXCL12, a chemokine involved in the regulated release of neutrophils from the bone marrow. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Taylor, S., et al. (2018). Front. Med. 5; 217; Sinha, S., et al. (1987). Proc. Natl. Acad. Sci. USA. 84(8); 2228-2232).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
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