推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1L16, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 20.53 kDa
observed mol wt ~18 kDa
純化經由
using Protein A
物種活性
mouse, human, rat
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1L16 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Bcl-2-binding component 3 (PUMA). It targets an epitope within 17 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Bcl-2-binding component 3 (PUMA).
應用
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PUMA in HepG2 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PUMA in NIH3T3 and L6 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound PUMA peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PUMA in human small intestine tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PUMA in A431, HeLa, HepG2, and NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PUMA in HepG2 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PUMA in NIH3T3 and L6 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound PUMA peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PUMA in human small intestine tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PUMA in A431, HeLa, HepG2, and NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Bcl-2-binding component 3, isoforms 1/2 (UniProt: Q9BXH1; also known as JFY-1, p53 up-regulated modulator of apoptosis. PUMA) is encoded by the BBC3 (also known as PUMA) gene (Gene ID: 27113) in human. PUMA is a member of the BH3-only Bcl-2 family that is localized in the mitochondria and serves as a critical mediator of p53-dependent and -independent apoptosis induced by a wide variety of stress stimuli and growth factor/cytokine withdrawal. It is normally expressed at a very low level in cells, however, it is rapidly induced under stress conditions. PUMA transduces death signals primarily to the mitochondria, where it acts indirectly on Bax and/or Bak by relieving the inhibition imposed by anti-apoptotic members. It is reported to bind and antagonize all antiapoptotic members of the Bcl-2 family and induce mitochondrial dysfunction, cytochrome c release, and caspase activation. Its BH3 domain, localized to amino acids 137-151, is required for its interaction with Bcl-2 like proteins. The BH3 domains forms an amphipathic a-helical structure that allows it to bind to anti-apoptotic Bcl-2 family proteins. Its C-terminal region contains a hydrophobic domain that directs its mitochondrial localization and both the BH3 domain and mitochondrial localization are shown to be essential induction of apoptosis. PUMA is considered as an essential mediator of p53-dependent and p53-independent apoptosis. Inhibition of PUMA can lead to apoptosis deficiency and increases the risk for cancer development and therapeutic resistance. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Yu, J., and Zhang, L. (2008). Oncogene. 27 (suppl. 1); S71-S83; Yu, J., et al. (2001). Mol Cell. 7(3); 673-682).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
商品
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
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