ZRB1553
Anti-phospho-mTOR-(Ser2448) Antibody, clone 1C22 ZooMAb® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells
别名:
Serine/threonine-protein kinase mTOR;EC:2.7.11.1;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;Mechanistic target of rapamycin;Rapamycin and FKBP12 target 1;Rapamy
About This Item
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生物来源
rabbit
质量水平
重组
expressed in HEK 293 cells
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
1C22, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
产品线
ZooMAb® learn more
表单
lyophilized
分子量
calculated mol wt 288.89 kDa
observed mol wt ~250 kDa
纯化方式
using Protein A
种属反应性
human, mouse
种属反应性(根据同源性预测)
monkey, rat
包装
antibody small pack of 25 μL
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
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增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技术
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型
IgG
表位序列
C-terminal
Protein ID登记号
UniProt登记号
环保替代产品分类
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
phosphorylation (pSer2448)
一般描述
特异性
免疫原
应用
Evaluated by Western Blotting in lysate from NIH3T3 cells treated with insulin.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected mTOR phosphorylated on serine 2448 in NIH3T3 cells, overnight serum stared and treated with insulin (500 nM for 15 min.).
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected phospho-mTOR-(Ser2448) in lysate from HEK293 cells overnight serum starved and treated with EGF (50 ng/mL; 10 min).
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-mTOR-(Ser2448) in human prostate cancer and human stomach cancer tissue sections.
Peptide Inhibition Assay: Target band detection in a lysate from NIH3T3 cells treated EGF (50 ng/mL; 10 min) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-mTOR-(Ser2448) in HEK293 cells overnight serum starved and treated with EGF (50 ng/mL; 10 min)..
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
外形
储存及稳定性
法律信息
免责声明
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储存分类代码
13 - Non Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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