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生物源
human
重組細胞
expressed in CHO cells
化驗
≥95% (HPLC)
≥95% (SDS-PAGE)
形狀
lyophilized
效力
≤10.0 ng/mL
分子量
17.9 kDa
包裝
pkg of 10 μg
技術
cell culture | mammalian: suitable
雜質
<0.1 EU/μg endotoxin, tested
顏色
white to off-white
適合性
suitable for molecular biology
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... FASLG(356)
一般說明
Fas Ligand (FasL) is a member of the TNF (tumor necrosis factor) superfamily that is expressed on the cell surface of activated T cells. It can be present as soluble form in the circulation or membrane bound form in cells. Recombinant human soluble Fas Ligand is a 17.9kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag. Both human and murine sFasL are fully active on human and murine cells.
應用
Fas ligand human has been used to induce apoptosis in T-cell lymphoma-derived HuT78 cells and jurkat cells.
生化／生理作用
Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-Associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppresses normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome.
序列
HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL
外觀
Lyophilized with no additives.
重構
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
The metalloproteinase matrilysin proteolytically generates active soluble Fas ligand and potentiates epithelial cell apoptosis.
Powell WC, et al.
Current Biology, 9, 1441-1447 (1999)
The molecular architecture of the TNF superfamily.
Bodmer JL, et al.
Trends in Biochemical Sciences, 27, 19-26 (2002)
Fas ligand mediates activation-induced cell death in human T lymphocytes.
Alderson MR, et al.
The Journal of Experimental Medicine, 71-77 (1995)
Karthik M Kodigepalli et al.
Cell cycle (Georgetown, Tex.), 16(2), 179-188 (2016-12-09)
Sterile α motif and HD domain-containing protein 1 (SAMHD1) is a mammalian dNTP hydrolase (dNTPase) that regulates intracellular dNTP balance. We have previously reported that SAMHD1 mRNA and protein levels are significantly downregulated in CD4
D H Lynch et al.
Immunity, 1(2), 131-136 (1994-05-01)
The gene for the mouse Fas ligand was cloned and its chromosomal position determined. Fasl was tightly linked to gld (no crossovers in 567 meiotic events) on mouse chromosome 1 and closely linked with a novel member of the same
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