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生物源
human
重組細胞
expressed in E. coli
化驗
≥90% (SDS-PAGE)
形狀
aqueous solution
分子量
59.5 kDa
包裝
pkg of 20 μg
儲存條件
avoid repeated freeze/thaw cycles
濃度
0.73 mg/mL
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... PTPN11(5781)
一般說明
Protein tyrosine phosphatase, non-receptor type 11 (PTPN11), also known as SHP-2, is a scaffolding protein. It possesses two SH2 domains at the amino terminal, a protein tyrosine phosphatase domain and carboxy-terminal region. The gene encoding SHP-2 is localized on human chromosome 12q24.13.
應用
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Human active SHP-2 has been used to study the effects of α-lipoic acid and dihydrolipoic acid on the activity of SHP-2 enzyme.
Human active SHP-2 has been used to study the effects of α-lipoic acid and dihydrolipoic acid on the activity of SHP-2 enzyme.
生化／生理作用
Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) or SHP-2 is involved in signal transduction pathways downstream of growth receptors. It acts as a proto-oncogene. SHP-2 leads to sustained RAS activation by dephosphorylating the RAS-GTPase-activating protein binding site on Grb2-associated binder 1 (Gab1). This protein also nucleates multiple signaling proteins and thus, acts as an adaptor protein. Mutations in the SHP-2 gene have been associated with Noonan syndrome.
單位定義
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
外觀
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
其他說明
Amino acids : 224-529
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
SHP2 is induced by the HBx-NF-?B pathway and contributes to fibrosis during human early hepatocellular carcinoma development.
Kang HJ
Oncotarget, 8(16) (2017)
Shp2 Plays a Critical Role in IL-6-Induced EMT in Breast Cancer Cells.
Sun X
International Journal of Molecular Sciences, 18(2) (2017)
Hotspots in PTPN11 Gene Among Indian Children With Noonan Syndrome.
Narayanan DL
Indian Pediatrics, 54(8), 638-643 (2017)
Genomic Duplication of PTPN11 is an Uncommon Cause of Noonan Syndrome
John M
American Journal of Medical Genetics. Part A (2009)
Naphak Modhiran et al.
Science translational medicine, 7(304), 304ra142-304ra142 (2015-09-12)
Complications arising from dengue virus infection include potentially fatal vascular leak, and severe disease has been linked with excessive immune cell activation. An understanding of the triggers of this activation is critical for the development of appropriately targeted disease control
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