SAE0111

磷酸酶(碱性) 来源于人类胎盘

≥10 units/mg solid (in glycine buffer)

• 别名: Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• CAS号: 9001-78-9
• EC 号: 3.1.3.1 (BRENDA, IUBMB)
• MDL號碼: MFCD00131849
• 分類程式碼代碼: 12352200
• NACRES: NA.54

属性

• 生物源: human placenta
• 比活性: ≥10 units/mg solid (in glycine buffer)
• UniProt登錄號: P05187
• 儲存溫度: −20°C
• 基因資訊: human ... ALPP(250)

说明

應用

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.

生化／生理作用

Alkaline phosphatase dephosphorylates proteins and nucleic acids. Human placental alkaline phosphatase (PLAP) has an unique residue, Glu-429. It is an allosteric enzyme that is uncompetitively inhibited by L-Phe, L-Trp, and L-Leu. PLAP is post-translationally modified. Phosphatidylinositol-specific phospholipase C may be involved in the release of PLAP. PLAP may have a role in cell division and may be involved in the transfer of maternal IgG to the fetus.

單位定義

One unit will hydrolyze 1.0 umole of P-Nitrophenyl Phosphate per minute at pH 10.4 at 37 Deg C.

安全信息

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H334
• 防範說明: P261 - P284 - P501
• 危險分類: Resp. Sens. 1
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable