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生物源
mouse
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
Profilin 1-3, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~15 kDa
物種活性
rat, bovine, human, mouse, canine
濃度
~1.0 mg/mL
技術
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using whole extracts of HeLa or NRK cells
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... PFN1(5216)
mouse ... Pfn1(18643)
rat ... Pfn1(64303)
一般說明
Monoclonal Anti-Profilin 1 (mouse IgG1 isotype) is derived from the hybridoma Profilin1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence located near the C-terminus of human profiling 1, conjugated to keyhole limpet hemocyanin (KLH). Profilin-1 (Pfn1), a major actin-regulatory protein is mapped to human chromosome17p13. It is the most ubiquitous and abundant protein and is highly expressed throughout development and adulthood in most tissues including brain.
免疫原
synthetic peptide corresponding to a sequence located near the C-terminus of human profilin 1, conjugated to KLH. The corresponding sequence is identical in mouse and rat profilin 1.
應用
Monoclonal Anti-Profilin 1 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.
生化／生理作用
Profilin-1 (Pfn1) is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Profilin 1 is a potent regulator of actin filament dynamics. It is suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when over expressed in breast cancer cells. Deletion of the profilin 1 gene leads to an embryonic lethal phenotype.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
PFN1 mutations are rare in Han Chinese populations with amyotrophic lateral sclerosis
Chen YP, et al.
Neurobiology of Aging, 34(7) (2013)
A balanced level of profilin-1 promotes stemness and tumor-initiating potential of breast cancer cells
Jiang C, et al.
Cell Cycle, 16(24) (2017)
Structure and functions of profilins
Krishnan K, et al.
Biophysical Reviews, 1(2), 71-71 (2009)
Sung Haeng Lee et al.
Molecules and cells, 29(4), 311-325 (2010-05-07)
The dynamic remolding of the actin cytoskeleton is a critical part of most cellular activities, and malfunction of cytoskeletal proteins results in various human diseases. The transition between two forms of actin, monomeric or G-actin and filamentous or F-actin, is
Profilin I is essential for cell survival and cell division in early mouse development
Witke W, et al.
Proceedings of the National Academy of Sciences of the USA, 98(7), 3832-3836 (2001)
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