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Merck

RAB0366

Sigma-Aldrich

小鼠 MMP-2 ELISA 试剂盒

for serum, plasma and cell culture supernatant

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.32

物種活性

mouse

包裝

kit of 96 wells (12 strips x 8 wells)

技術

ELISA: suitable
capture ELISA: suitable

輸入

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 27.43-20000 pg/mL

檢測方法

colorimetric

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

mouse ... Mmp2(17390)

一般說明

Matrix Metallopeptidase 2 (MMP-2), also known as gelatinase A, belongs to the gelatinase subclass of the matrix metalloproteinase (MMP) family. It contains zinc in the active site. MMP-2 cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, vitronectin, elastin, laminin, proteoglycans, and a range of non-extracellular matrix (ECM) components. Both MMP-2 and MMP9 are important for matrix degradation and they are implicated in the maintenance of neovascularization. The Mouse MMP-2 enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP-2 in serum, plasma, cell culture supernatants, and urine.

免疫原

Recombinant Mouse MMP2

應用

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse MMP-2 ELISA Kit has been used in enzyme-linked immunosorbent assay to quantify matrix metalloproteinase -2 (MMP-2) secreted in supernatants by gold nanoparticles (AuNP)-loaded endothelial cells and superparamagnetic iron oxide nanoparticle (SPION) loaded endothelial cells.

其他說明

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)化学品安全说明书

  • RABSTOP3ELISA Stop Solution (Item I)化学品安全说明书

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)化学品安全说明书

  • RABWASH420X Wash Buffer (Item B)化学品安全说明书

象形圖

Corrosion

訊號詞

Warning

危險聲明

防範說明

危險分類

Met. Corr. 1

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Takayuki Shiomi et al.
Pathology international, 60(7), 477-496 (2010-07-03)
Cellular functions within tissues are strictly regulated by the tissue microenvironment which comprises extracellular matrix and extracellular matrix-deposited factors such as growth factors, cytokines and chemokines. These molecules are metabolized by matrix metalloproteinases (MMP), a disintegrin and metalloproteinases (ADAM) and
Subhadeep Chakrabarti et al.
Experimental lung research, 31(6), 599-621 (2005-07-16)
The lung is affected by a variety of disease processes that can lead to considerable morbidity and mortality. As the lung is the only organ for respiration and gas exchange, the structural and functional integrity of the lung is of
Disturbance of adhesomes by gold nanoparticles reveals a size- and cell type-bias.
Mulens-Arias, et al.
Biomaterials Science, 7, 389-408 (2019)
Theranostic iron oxide nanoparticle cargo defines extracellular vesicle-dependent modulation of macrophage activation and migratory behavior
Mulens-Arias, V, et al.
Advanced Biosystems, 1800079-1800079 (2018)
J C Rodriguez-Manzaneque et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(22), 12485-12490 (2001-10-19)
Growth of tumors and metastasis are processes known to require neovascularization. To ascertain the participation of the endogenous angiogenic inhibitor thrombospondin-1 (TSP1) in tumor progression, we generated mammary tumor-prone mice that either lack, or specifically overexpress, TSP1 in the mammary

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