P5367

Anti-Par-4 (Prostate Apoptosis Response-4) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL编号: MFCD03791926
• UNSPSC代码: 12352203
• NACRES: NA.46

属性

• 生物来源: rabbit
• 质量水平: 200
• 偶联物: unconjugated
• 抗体形式: IgG fraction of antiserum
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 表单: buffered aqueous solution
• 分子量: antigen 38 kDa
• 种属反应性: human, rat, mouse
• 技术: microarray: suitable; western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line; western blot: 1:5,000 using whole cell extract of the human HeLa cell line
• UniProt登记号: Q96IZ0
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... PAWR(5074) ; mouse ... Pawr(114774) ; rat ... Pawr(64513)

说明

一般描述

PRKC apoptosis WT1 regulator protein (PAWR) is also known as prostate apoptosis response 4 protein (Par-4). The PAWR gene is mapped to human chromosome 12q21.2. It is a 40 kDa protein expressed ubiquitously in mammals. It was first identified in prostate tumors.It has a leucine zipper domain and nuclear localization sequences. A core domain comprising of region 137-195 amino acids is critical for the apoptotic function of PAR-4. It interacts and modulates the activity of protein kinase C.

免疫原

synthetic peptide corresponding to the C-terminus of human Par-4 (amino acids 324-342) conjugated to KLH.

应用

Anti-Par-4 (Prostate Apoptosis Response-4) antibody produced in rabbit may be used in immunoblotting.

生化/生理作用

Par-4 protein interacts with Wilm′s tumor suppressor WT1 and modulates its transcription and growth suppression function. The gene for Par-4 is down-regulated by oncogenic Ras via the Raf-1- mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK-ERK) pathway in immortalized fibroblasts. Overexpression of the PAR4 gene causes down-regulation of B-cell lymphoma-2 (Bcl-2) in fibroblasts and prostate cancer cells. Par-4 protein plays an important role in neuronal apoptosis. This gene is upregulated in neuronal degeneration and is observed in Alzheimer′s disease.

The primary function of prostate apoptosis response 4 protein (Par-4) is to mediate apoptosis. In neuronal progenitor cells, ceramide and PAR-4 contribute to rapid apoptosis. PAR-4 activates the tumor necrosis factor receptor superfamily member (FAS)-based death signalling caspase cascade to accomplish apoptosis in prostate tumor cells. PAR-4 is considered as a potential drug for treatment of cancer and neurodegenerative disorders.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.