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生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 38 kDa
种属反应性
human, rat, mouse
技术
microarray: suitable
western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line
western blot: 1:5,000 using whole cell extract of the human HeLa cell line
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... PAWR(5074)
mouse ... Pawr(114774)
rat ... Pawr(64513)
一般描述
PRKC apoptosis WT1 regulator protein (PAWR) is also known as prostate apoptosis response 4 protein (Par-4). The PAWR gene is mapped to human chromosome 12q21.2. It is a 40 kDa protein expressed ubiquitously in mammals. It was first identified in prostate tumors.It has a leucine zipper domain and nuclear localization sequences. A core domain comprising of region 137-195 amino acids is critical for the apoptotic function of PAR-4. It interacts and modulates the activity of protein kinase C.
免疫原
synthetic peptide corresponding to the C-terminus of human Par-4 (amino acids 324-342) conjugated to KLH.
应用
Anti-Par-4 (Prostate Apoptosis Response-4) antibody produced in rabbit may be used in immunoblotting.
生化/生理作用
Par-4 protein interacts with Wilm′s tumor suppressor WT1 and modulates its transcription and growth suppression function. The gene for Par-4 is down-regulated by oncogenic Ras via the Raf-1- mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK-ERK) pathway in immortalized fibroblasts. Overexpression of the PAR4 gene causes down-regulation of B-cell lymphoma-2 (Bcl-2) in fibroblasts and prostate cancer cells. Par-4 protein plays an important role in neuronal apoptosis. This gene is upregulated in neuronal degeneration and is observed in Alzheimer′s disease.
The primary function of prostate apoptosis response 4 protein (Par-4) is to mediate apoptosis. In neuronal progenitor cells, ceramide and PAR-4 contribute to rapid apoptosis. PAR-4 activates the tumor necrosis factor receptor superfamily member (FAS)-based death signalling caspase cascade to accomplish apoptosis in prostate tumor cells. PAR-4 is considered as a potential drug for treatment of cancer and neurodegenerative disorders.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Identification of a unique core domain of par-4 sufficient for selective apoptosis induction in cancer cells
El-Guendy N, et al.
Molecular and Cellular Biology, 23(16), 5516-5525 (2003)
Structural and functional characterization of the upstream regulatory region of the human gene encoding prostate apoptosis response factor-4
Hsu SC, et al.
Gene, 295(1), 109-116 (2002)
Regulation of cell death in mitotic neural progenitor cells by asymmetric distribution of prostate apoptosis response 4 (PAR-4) and simultaneous elevation of endogenous ceramide
Bieberich E, et al.
The Journal of Cell Biology, 162(3), 469-479 (2003)
M Barradas et al.
The EMBO journal, 18(22), 6362-6369 (1999-11-24)
Inhibition of apoptosis is an important characteristic of oncogenic transformation. The Par-4 gene product has recently been shown to be upregulated in cells undergoing apoptotic cell death, and its ectopic expression was shown to be critical in apoptosis. We demonstrate
S G Qiu et al.
Oncogene, 18(50), 7115-7123 (1999-12-22)
Oncogenic variants of the cellular Ras protein are often associated with different types of human cancers. However, the mechanisms by which oncogenic Ras induces transformation are not fully established. Expression of the transcriptional repressor Par-4 was down-regulated by oncogenic Ras
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