OGS3164

PSF-OXB20-NH2-TORA - TORA SECRETION PLASMID

plasmid vector for molecular cloning

• 别名: cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• 分類程式碼代碼: 12352200

属性

• 重組細胞: expressed in E. coli
• 形狀: buffered aqueous solution
• 分子量: size 3965 bp
• 菌種選擇: kanamycin
• 複製起點: pUC (500 copies)
• 肽切割: no cleavage
• 肽標籤位置: N-terminal
• 啟動子: Promoter name: OXB20; Promoter activity: constitutive; Promoter type: bacterial
• 報告基因: none
• 分泌信號: TorA
• 運輸包裝: ambient
• 儲存溫度: −20°C

说明

一般說明

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains an TorA secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. .

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

序列

To view sequence information for this product, please visit the product page

分析報告

To view the Certificate of Analysis for this product, please visit www.oxgene.com

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable